Journal: Plant Physiology

Article Title: The Grapevine R2R3-MYB Transcription Factor VvMYBF1 Regulates Flavonol Synthesis in Developing Grape Berries 1 [W] [OA]

doi: 10.1104/pp.109.142059

Figure Lengend Snippet: Light induces expression of VvMYBF1 and VvFLS1 and subsequent flavonol synthesis in Chardonnay cells. Transcript levels were determined by qPCR and are shown relative to VvUbiquitin1 (TC32075) expression. Each data point represents the mean of three replicate PCRs with error bars indicating se. A, VvMYBF1 expression. B, VvFLS1 transcript levels in Chardonnay cell samples exposed to light. C, Accumulation of total flavonols in Chardonnay cell samples exposed to light (gray bars) and kept in the dark (black bars) determined by HPLC analysis. D, Positions of putative cis-regulatory elements in the promoters of VvMYBF1 and VvFLS1 from Shiraz, Pinot Noir, and Chardonnay relative to the transcriptional start codon. The putative transcriptional start sites are referred to as positions +1, while the translational start codons are marked by the bent arrows. Numbers above the boxes indicate the distance from the transcriptional start. ACS, ACGT-containing sequence similar to ACE in the Arabidopsis CHS promoter (accession no. S000355); MRS, MYB recognition sequence similar to MRE in the Arabidopsis CHS promoter (S000356); RRS, R response sequence similar to RRE in the Arabidopsis CHS promoter (S000407); IBOXCORE element (S000199), LREBOXIPCCHS1 consensus sequence (S000302), MYBCORE (S000176).

Article Snippet: HPLC analysis was performed on total methanolic extracts of Col-0, myb12 and myb11-12-111 mutant lines, and complemented lines on a reverse-phase HPLC device (Kontron Instruments; 322 pump system/360 autosampler/335 HPLC detector) with a Symmetry C18 column (3.5 μ m, 4.6 × 150 mm [WAT200632]; Waters) protected by a guard column.

Techniques: Expressing, Sequencing

Journal: Plant Physiology

Article Title: The Grapevine R2R3-MYB Transcription Factor VvMYBF1 Regulates Flavonol Synthesis in Developing Grape Berries 1 [W] [OA]

doi: 10.1104/pp.109.142059

Figure Lengend Snippet: Flavonol accumulation in Arabidopsis seedlings. A to D, Seedlings were stained with the flavonol-specific dye DPBA. Flavonol staining in Arabidopsis seedlings was visualized using epifluorescence microscopy. Images of representative seedlings of Col-0 (A), Col-0 expressing VvMYBF1 (Col-0/proAtMYB12∷VvMYBF1; B), myb12 mutants (C), and one representative complemented line expressing VvMYBF1 (myb12/proAtMYB12∷VvMYBF1; D) are shown. Bars = 0.1 cm. E, Comparison of the relative flavonol content of methanolic extracts of Arabidopsis seedlings by HPLC analysis. The results are from one representative experiment that was repeated with similar relative values. FW, Fresh weight.

Article Snippet: HPLC analysis was performed on total methanolic extracts of Col-0, myb12 and myb11-12-111 mutant lines, and complemented lines on a reverse-phase HPLC device (Kontron Instruments; 322 pump system/360 autosampler/335 HPLC detector) with a Symmetry C18 column (3.5 μ m, 4.6 × 150 mm [WAT200632]; Waters) protected by a guard column.

Techniques: Staining, Epifluorescence Microscopy, Expressing, Comparison